So.. what I'm interested in.. is what kind of like his chromosome creates..

If I'm not mistaken, though I probably am.. been a while since I was in a biology class but.. humans dont have 381 pairs of gene code..

A chromosome made of 381 genes, not 381 chromosomes !
The DNA of the most simple form of life is made of 500+ genes thus Mycoplasma is really a brand new living entity, not just a mutation
Some part of the chromosome are in any case taken ( or copied ) by natural micro organisms
About the application
Craig Venter is researching an efficent system to turn C02 into 02
in practice, a super "cleaner "

and that leaves C? This carbon particle forms the fuel of the entity then? Does it convert carbon in something else or does it use carbon for splitting itself or so? Does this cell actually split at all?

well I dont know these details
The new chromosone has not been yet injected in the cell so it is not yet proved that it can reproduce itself and that it can perform the task for what has been created
However scientists dont doubt that it is just a matter of time
we will see.
If the new artificial micro organism should also be able to evolve, by itself,in a more sophisticated micro organism, suitable , for example, to survive in a tougher enviromental condition than it would be hard, in my opinion, to claim that a creator is really needed

Well sure a creator is needed to create the first chromosome, and these strings of DNA keep the same when the cell multiplies so the source code of all chromosomes is exactly the same as the original one and property of the creator

In a not so distant future chromosomes appear for the judge because of illegal copy of copyrighted material

bad joke.